Journal: PLoS ONE
Article Title: Visualization and Curve-Parameter Estimation Strategies for Efficient Exploration of Phenotype Microarray Kinetics
doi: 10.1371/journal.pone.0034846
Figure Lengend Snippet: The upper panels show the plot of the respiration curves of E. coli DSM 18039 = K12 on well C08 when grown on solid LB medium for 16.75 h (t 1 ), 18 h (t 2 ), 19.33 (t 3 ), 20.5 (t 4 ), 21.92 (t 5 ), 23.25 h (t 6 ), 24.5 h (t 7 ), 25.58 h (t 8 ) or 40.33 h (t 9 ), respectively, and subsequently measured on GEN III microplates™ in the PM modus over 91 h. The lower panels shows point estimates and 95% CIs for each of the four parameters from the spline approach. The blue lines highlight the position of the upper and lower limits of the CIs from repetition no. 4 at t 5 . A non-overlap of the CIs of different curves indicates a difference of a statistically detectable amount, and the distance between two intervals provides information about the expected minimum difference.
Article Snippet: To ensure that all ten replicate plates were inoculated with cells of identical physiological conditions, the desired cell concentration was adjusted in a pool of ten vials of GEN III inoculation medium A (AES Chemunex BLG 72401) which was then simultaneously inoculated into ten GEN III plates.
Techniques: